The Flow Cytometry & Cell Sorting (FCCS) Shared Resource Facility is co-sponsored as a Hollings Cancer Center (HCC) Core Facility and a University Research Resources Facility. Equipped with state-of-the-art instruments and staffed by experts, the goal of the FCCS Shared Resource is to provide comprehensive analytic flow cytometry and high speed cell sorting services in a cost-effective manner to HCC members. The staff of the resource have considerable expertise in high-speed sorting of rare populations of cells including stem/progenitor cells and epitope-specific T cells and are continually expanding the repertoire of available techniques to meet the needs of the innovative research within the HCC.

The HCC FCCS Shared Resource offers a wide range of services from access to routine flow cytometric analysis to expertise in high-speed cell sorting to the development of novel assays. Examples of assays available include, but are not limited to, the following: immunophenotyping, cell cycle analysis, DNA ploidy analysis, apoptosis, cell proliferation (BrdU incorporation), intracellular antigen/protein and membrane potential measurement, as well as cytokine detection (bead assay). High-speed cell sorting based on cell surface marker immunostaining and/or side-population staining is also available.